RECENT ADVANCES IN UV-VISIBLE SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION FOR PHARMACEUTICAL DRUG ANALYSIS

ARTICLE HISTORY: Submission: Jan 20, 2026; Revision: Feb 08, 2026; Acceptance: Feb 25, 2026; DOI INFO: https://doi.org/10.56815/ijmsci.v6i1.2026.1-16

Abstract

This work set out to meet the requirements of ICH Q2(R1) for the quantitative assessment of paracetamol in both its pure form and its commercial tablet forms by creating and validating a simple, precise, accurate, and cost-effective UV-Visible spectrophotometric technique. The absorbance measurements were taken using a double-beam Shimadzu UV-1800 spectrophotometer at 257 nm, with 0.1 N sodium hydroxide serving as the solvent in the procedure. Over the linear concentration range of 5-25 µg/ml, the created technique complied with Beer-Lambert's law, producing a calibration curve with a remarkable correlation coefficient (r²) of 0.9995. The technique's linearity, accuracy, precision, repeatability, intra-day, and inter-day performance, as well as its limit of detection (LOD) and limit of quantitation (LOQ) were all thoroughly tested, and the method was also tough and durable. The recovery tests were conducted at 80%, 100%, and 120% levels of accuracy. The method's dependability and repeatability were confirmed by recovery values that were within the permitted range of 98-102% and %RSD values for precision studies that stayed below 2%. Calpol 650 mg, Calpol 500 mg, and Dolo 650 mg were found to have percentage purity findings that fell within the labeled claim by 98-102% when the test was conducted utilizing absorptivity, single-point standard comparison, and calibration curve techniques. The suggested approach is ideal for the regular quality control analysis of paracetamol in pharmaceutical tablet dosage forms and bulk medicinal substances since it is simple, quick, cheap, and does not interact with common excipients.